Plasmid

Part:BBa_J119399:Design

Designed by: Andrew Hunn   Group: Eckdahl Lab   (2016-07-18)


RS10 in J100270 Selection Phage


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5551
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2016
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5551
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5551
    Illegal NgoMIV site found at 8513
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Inserted riboswitch via decoupled golden gate assembly.


Source

Wachsmuth et. al., J100263

References

Christy Enriquez annotated and edited.